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Image Search Results
Journal: Open Life Sciences
Article Title: Constitutive and evoked release of ATP in adult mouse olfactory epithelium
doi: 10.1515/biol-2022-0811
Figure Lengend Snippet: Immature OSN, mature OSN, sustentacular cells, and microvillous cells are present in 7–8 DIV adult OE primary epithelium cell cultures. (a–d). Representative grayscale pictures of cells present in adult primary olfactory cell cultures. Each panel corresponds to a separate immunocytochemistry experiment testing one antibody and showing cells immuno-positive for the tested antibody. (a) Use of antibody against neuronal precursor marker nestin, (b) use of antibody against sustentacular cell marker calnexin, (c) use of antibody against mature olfactory neuron marker OMP, and (d) use of antibody against microvillous cell marker PLCβ2. Scale bar = 20 µm. In each panel, each arrow indicates a single immunopositive cell; n = 3 coverslips for each tested antibody.
Article Snippet: Primary cell cultures (7–8 DIV) were processed for immunoreactivity to rabbit anti-calnexin (1:500; Santa Cruz Biotechnology, Santa Cruz, CA), mouse anti-nestin (1:1,000; Millipore, Billerica, MA),
Techniques: Immunocytochemistry, Marker